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Bovine MMP13 (Matrix Metalloproteinase 13) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MMP-13 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MMP-13 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MMP-13 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EB0160
- Alias:
- Collagenase 3 ELISA Kit , Matrix metalloproteinase-13 ELISA Kit , MMP-13 ELISA Kit , MMP13 ELISA Kit
- Gene Name:
- MMP13
- UniProt ID:
- O77656
- Product Name
- Bovine MMP13 (Matrix Metalloproteinase 13) ELISA Kit
- Catalogue No.
- EB0160
- Gene Name
- MMP13
- UniProt ID
- O77656
- Alias
- Collagenase 3 ELISA Kit , Matrix metalloproteinase-13 ELISA Kit , MMP-13 ELISA Kit , MMP13 ELISA Kit
- Sample Type
- 血清，血浆，细胞培养上清液，细胞裂解液或组织裂解液，其它生物液体样品
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4小时
- Range
- 0.156-10ng/ml
- Sensitivity
- 0.094ng/ml
- Specificity
- Specifically binds with MMP-13 , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E008 Matrix Elimination Agent A 1vial 1.5ml 2vial 1.5ml E009 Matrix Elimination Agent B 1vial 1.5ml 2vial 1.5ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.093 0.095 0.094 0.156 0.181 0.187 0.184 0.312 0.367 0.377 0.372 0.625 0.454 0.468 0.461 1.25 0.733 0.755 0.744 2.5 1.18 1.214 1.197 5 1.785 1.837 1.811 10 2.709 2.787 2.748 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (ng/ml) 0.29 1.24 5.03 0.31 Inter-assay Precision Standard deviation 0.02 0.07 0.24 0.02 Inter-assay Precision CV(%) 5.91 5.26 4.68 5.22
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EB0160 — Bovine MMP13 (Matrix Metalloproteinase 13) ELISA Kit