Products
Bovine AMH (Muellerian-inhibiting factor) ELISA kit
This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with AMH. AMH in the sample or standard competes with a fixed amount of biotin-labeled AMH for sites on a pre-coated antibody specific to AMH. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of AMH in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
- Catalogue No.:
- EB0213
- Alias:
- Muellerian-inhibiting factor ELISA Kit , Anti-Muellerian hormone ELISA Kit , AMH ELISA Kit , Muellerian-inhibiting substance ELISA Kit , MIS ELISA Kit , AMH ELISA Kit , MIF ELISA Kit
- Gene Name:
- AMH
- UniProt ID:
- P03972
- Product Name
- Bovine AMH (Muellerian-inhibiting factor) ELISA kit
- Catalogue No.
- EB0213
- Gene Name
- AMH
- UniProt ID
- P03972
- Alias
- Muellerian-inhibiting factor ELISA Kit , Anti-Muellerian hormone ELISA Kit , AMH ELISA Kit , Muellerian-inhibiting substance ELISA Kit , MIS ELISA Kit , AMH ELISA Kit , MIF ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Competitive ELISA, Coated with Antigen
- Detection Wavelength
- OD450
- Reaction Duration
- 2 hours
- Range
- 15.625-1000pg/ml
- Sensitivity
- 9.375pg/ml
- Specificity
- Specifically binds with AMH , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Lyophilized Biotin-labeled Antibody(Concentrated) 1vial 1vial E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E005 Purified Water 200ul 200ul 2-8°C E039 Sample Dilution Buffer 10ml 20ml E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 2.124 2.186 2.155 15.625 1.415 1.457 1.436 31.25 1.029 1.059 1.044 62.5 0.632 0.65 0.641 125 0.443 0.455 0.449 250 0.268 0.276 0.272 500 0.203 0.209 0.206 1000 0.103 0.105 0.104 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 32.11 123.1 483 32.41 Inter-assay Precision Standard deviation 1.54 6.82 24.83 1.58 Inter-assay Precision CV(%) 4.8 5.54 5.14 4.87
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
How do I place an order?
What is the dispatch time?
Do you ship outside Türkiye?
How should an unopened kit be stored?
How long is an opened kit stable?
What should I do if the shipment arrives warm?
Which sample types can I use?
My background is too high. What should I check?
Can I run fewer wells than the kit provides?
Something did not work. What do you need from me?
Do you provide a certificate of analysis?
Aradığınızı bulamadınız mı? Tüm SSS sayfasına bakın veya bize yazın.
Related Products
Product Inquiry
EB0213 — Bovine AMH (Muellerian-inhibiting factor) ELISA kit