Products
Canine IL-12/P70 (Interleukin 12 (P70)) ELISA Kit
Category:
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-12/P70 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-12/P70 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-12/P70 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- ECA0034
- Alias:
- Interleukin-12 subunit alpha ELISA Kit , IL-12A ELISA Kit , Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit , CLMF p35 ELISA Kit , IL-12 subunit p35 ELISA Kit , IL12A ELISA Kit , Interleukin-12 subunit beta ELISA Kit , IL-12B ELISA Kit , Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit , CLMF p40 ELISA Kit , IL-12 subunit p40 ELISA Kit , IL12B ELISA Kit
- Gene Name:
- IL-12/P70
- UniProt ID:
- Q28267 Q28268
- Product Name
- Canine IL-12/P70 (Interleukin 12 (P70)) ELISA Kit
- Catalogue No.
- ECA0034
- Gene Name
- IL-12/P70
- UniProt ID
- Q28267 Q28268
- Alias
- Interleukin-12 subunit alpha ELISA Kit , IL-12A ELISA Kit , Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit , CLMF p35 ELISA Kit , IL-12 subunit p35 ELISA Kit , IL12A ELISA Kit , Interleukin-12 subunit beta ELISA Kit , IL-12B ELISA Kit , Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit , CLMF p40 ELISA Kit , IL-12 subunit p40 ELISA Kit , IL12B ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 15.625-1000pg/ml
- Sensitivity
- 9.375pg/ml
- Specificity
- Specifically binds with IL-12/P70 , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.078 0.08 0.079 15.625 0.164 0.168 0.166 31.25 0.25 0.258 0.254 62.5 0.381 0.393 0.387 125 0.656 0.676 0.666 250 0.967 0.995 0.981 500 1.529 1.573 1.551 1000 2.069 2.129 2.099 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 30.88 124.4 497 31.41 Inter-assay Precision Standard deviation 1.47 6.46 25.45 1.49 Inter-assay Precision CV(%) 4.76 5.19 5.12 4.73
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.
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ECA0034 — Canine IL-12/P70 (Interleukin 12 (P70)) ELISA Kit