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Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MMP-2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MMP-2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MMP-2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- ECA0047
- Alias:
- 72 kDa type IV collagenase ELISA Kit , 72 kDa gelatinase ELISA Kit , Gelatinase A ELISA Kit , Matrix metalloproteinase-2 ELISA Kit , MMP-2 ELISA Kit , TBE-1 ELISA Kit , MMP2 ELISA Kit , CLG4A ELISA Kit
- Gene Name:
- MMP2
- UniProt ID:
- Q9N1P6
- Product Name
- Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit
- Catalogue No.
- ECA0047
- Gene Name
- MMP2
- UniProt ID
- Q9N1P6
- Alias
- 72 kDa type IV collagenase ELISA Kit , 72 kDa gelatinase ELISA Kit , Gelatinase A ELISA Kit , Matrix metalloproteinase-2 ELISA Kit , MMP-2 ELISA Kit , TBE-1 ELISA Kit , MMP2 ELISA Kit , CLG4A ELISA Kit
- Sample Type
- 血清，血浆，细胞培养上清液，细胞裂解液或组织裂解液，其它生物液体样品
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4小时
- Range
- 31.25-2000pg/ml
- Sensitivity
- 18.75pg/ml
- Specificity
- Specifically binds with MMP-2 , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E008 Matrix Elimination Agent A 1vial 1.5ml 2vial 1.5ml E009 Matrix Elimination Agent B 1vial 1.5ml 2vial 1.5ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.1 0.102 0.101 31.25 0.224 0.23 0.227 62.5 0.351 0.361 0.356 125 0.524 0.54 0.532 250 0.831 0.855 0.843 500 1.344 1.384 1.364 1000 1.567 1.613 1.59 2000 2.324 2.392 2.358 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 60.53 257 990.1 64 Inter-assay Precision Standard deviation 2.83 12.57 52.08 3.33 Inter-assay Precision CV(%) 4.67 4.89 5.26 5.21
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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My background is too high. What should I check?
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ECA0047 — Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit