Human GAL3(Galectin-3) ELISA Kit

References #13
Category:

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GAL3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with GAL3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of GAL3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
EH0145
Alias: 
Galectin-3 ELISA Kit , Gal-3 ELISA Kit , 35 kDa lectin ELISA Kit , Carbohydrate-binding protein 35 ELISA Kit , CBP 35 ELISA Kit , Galactose-specific lectin 3 ELISA Kit , Galactoside-binding protein ELISA Kit , GALBP ELISA Kit , IgE-binding protein ELISA Kit , L-31 ELISA Kit , Laminin-binding protein ELISA Kit , Lectin L-29 ELISA Kit , Mac-2 antigen ELISA Kit , LGALS3 ELISA Kit , MAC2 ELISA Kit
Gene Name: 
GAL3
UniProt ID: 
P17931
Manual MSDS
Size Price
48T/96T Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
Human GAL3(Galectin-3) ELISA Kit
Catalogue No.
EH0145
Gene Name
GAL3
UniProt ID
P17931
Alias
Galectin-3 ELISA Kit , Gal-3 ELISA Kit , 35 kDa lectin ELISA Kit , Carbohydrate-binding protein 35 ELISA Kit , CBP 35 ELISA Kit , Galactose-specific lectin 3 ELISA Kit , Galactoside-binding protein ELISA Kit , GALBP ELISA Kit , IgE-binding protein ELISA Kit , L-31 ELISA Kit , Laminin-binding protein ELISA Kit , Lectin L-29 ELISA Kit , Mac-2 antigen ELISA Kit , LGALS3 ELISA Kit , MAC2 ELISA Kit
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
4 hours
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Specificity
Specifically binds with GAL3 , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
E001ELISA Microplate(Dismountable) 8×68×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002Lyophilized Standard 1vial2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E003Biotin-labeled Antibody(Concentrated, 100X) 60ul120ul 2-8°C (Avoid Direct Light)
E034HRP-Streptavidin Conjugate(SABC, 100X) 60ul120ul
E024TMB Substrate 5ml10ml
E039Sample Dilution Buffer 10ml20ml 2-8°C
E040Antibody Dilution Buffer 5ml10ml
E049SABC Dilution Buffer 5ml10ml
E026Stop Solution 5ml10ml
E038Wash Buffer(Concentrated, 25X) 15ml30ml
E006Plate Sealer 3 pieces5 pieces
E007Product Description 1 copy1 copy
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
00.0930.0970.095
0.1560.1230.1290.126
0.3120.2180.2290.223
0.6250.3590.3780.367
1.250.6080.6390.621
2.51.0021.0531.023
51.621.7031.654
102.1232.2322.167
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (ng/ml)0.291.245.060.28
Inter-assay Precision Standard deviation0.020.060.210.02
Inter-assay Precision CV(%)5.224.964.235.43


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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Cited Publications (13)

Machine learning reveals an interleukin-33-associated immuno-metabolic axis defining recovery endotypes in severe combat trauma IF: 7

Frontiers in Immunology · 2026-08-21

Epigenetic Regulation of Galectin-1 and Galectin-3 in Osteoporosis: A Pilot Study in Patients Undergoing Total Joint Arthroplasty IF: 6

Cells · 2026-07-03

Serum Fetuin A, Galectin-3 and BMI correlation in GDM vs. healthy pregnant women at an Indian Tertiary Center IF: 7.4

Diabetes Research and Clinical Practice · 2025-12-26

Clinical and prognostic significance of biomarker ratios in patients with heart failure and concomitant atrial fibrillation and diabetes mellitus

International Journal of Endocrinology (Ukraine) · 2025-12-26

Effect of Dapagliflozin on Oxidative Stress in Patients with Heart Failure

University of Thi-Qar Journal of Science · 2025-12-12

The value of biomarkers in predicting the course of heart failure of ischemic genesis in patients with atrial fibrillation and diabetes mellitus

Wiadomo?ci Lekarskie Medical Advances · 2025-10-17

The Role of Diastolic Stress Echo and Myocardial Work in Early Detection of Cardiac Dysfunction in Women with Breast Cancer Undergoing Chemotherapy IF: 3.9

Biomedicines · 2025-10-11

The Diagnostic Role of Novel Echocardiography Indices and Arterial Stiffness in Diabetic Cardiomyopathy IF: 3.9

Biomedicines · 2025-09-26

The silent predictors: exploring galectin-3 and Irisin's tale in severe COVID-19 IF: 1.6

BMC Research Notes · 2024-11-08

Study of Serum Gremlin-1, Galectin-3, and Oxyntomodulin in Women with Breast Cancer

Pakistan Journal of Life and Social Sciences · 2024-07-05

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