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Human APC(Adenomatous polyposis coli protein) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti APC antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with APC bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of APC in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EH1239
- Alias:
- Adenomatous polyposis coli protein ELISA Kit , Protein APC ELISA Kit , Deleted in polyposis 2.5 ELISA Kit , APC ELISA Kit , DP2.5 ELISA Kit
- Gene Name:
- Human
- UniProt ID:
- P25054
- Product Name
- Human APC(Adenomatous polyposis coli protein) ELISA Kit
- Catalogue No.
- EH1239
- Gene Name
- Human
- UniProt ID
- P25054
- Alias
- Adenomatous polyposis coli protein ELISA Kit , Protein APC ELISA Kit , Deleted in polyposis 2.5 ELISA Kit , APC ELISA Kit , DP2.5 ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 15.625-1000pg/ml
- Sensitivity
- 9.375pg/ml
- Specificity
- Specifically binds with APC , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.102 0.104 0.103 15.625 0.152 0.156 0.154 31.25 0.211 0.217 0.214 62.5 0.302 0.31 0.306 125 0.448 0.462 0.455 250 0.806 0.83 0.818 500 1.353 1.393 1.373 1000 2.036 2.096 2.066 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 32.28 124.4 499 32.81 Inter-assay Precision Standard deviation 1.61 6.23 26.15 1.71 Inter-assay Precision CV(%) 4.99 5.01 5.24 5.21
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EH1239 — Human APC(Adenomatous polyposis coli protein) ELISA Kit