Products
Mouse F II(coagulation factor II) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti F II antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with F II bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of F II in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EM1012
- Alias:
- Prothrombin ELISA Kit , Coagulation factor II ELISA Kit , Activation peptide fragment 1 ELISA Kit , Activation peptide fragment 2 ELISA Kit , Thrombin light chain ELISA Kit , Thrombin heavy chain ELISA Kit , F2 ELISA Kit
- UniProt ID:
- P19221
- Product Name
- Mouse F II(coagulation factor II) ELISA Kit
- Catalogue No.
- EM1012
- UniProt ID
- P19221
- Alias
- Prothrombin ELISA Kit , Coagulation factor II ELISA Kit , Activation peptide fragment 1 ELISA Kit , Activation peptide fragment 2 ELISA Kit , Thrombin light chain ELISA Kit , Thrombin heavy chain ELISA Kit , F2 ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 0.156-10ng/ml
- Sensitivity
- 0.094ng/ml
- Specificity
- Specifically binds with F II , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.141 0.145 0.143 0.156 0.217 0.223 0.22 0.312 0.407 0.419 0.413 0.625 0.707 0.727 0.717 1.25 1.194 1.228 1.211 2.5 1.535 1.579 1.557 5 1.816 1.868 1.842 10 2.105 2.167 2.136 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (ng/ml) 0.32 1.27 5.23 0.31 Inter-assay Precision Standard deviation 0.02 0.07 0.26 0.02 Inter-assay Precision CV(%) 6.32 5.86 4.95 6.2
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EM1012 — Mouse F II(coagulation factor II) ELISA Kit