Annexin V-APC/DAPI Apoptosis Kit

Annexin V-APC/DAPI apoptosis detection kit can be used to detect apoptosis in suspension cells and adherent cells. Annexin V is a calcium-dependent phosphatidylserine binding protein with a high affinity for phosphatidylserine PS. Annexin V labelled APC can bind to the membrane of early apoptotic cells by means of the PS exposed outside the cells. Apoptosis can be detected by flow cytometry or fluorescence microscopy. Cell membrane integrity is lost due to late apoptosis or necrosis, and 4',6-Diamidino-2-Phenylindole dihydrochloride (4', 6-Diamidino-2-phenylindole, DAPI) can bind specifically to double-stranded DNA and produce strong fluorescence. Cells at different apoptotic stages can be distinguished.