Annexin V-PE/7-AAD Apoptosis Kit

Annexin V-PE/7-AAD apoptosis detection kit can be used to detect apoptosis in suspension cells and adherent cells. Annexin V is a calcium-dependent phosphatidylserine binding protein with a high affinity for phosphatidylserine PS. Annexin V labelled PE can bind to the membrane of early apoptotic cells by means of the PS exposed outside the cells. Apoptosis can be detected by flow cytometry or fluorescence microscopy. 7-AAD binds specifically to double-stranded DNA and produces strong fluorescence, which is normally unable to penetrate cell membranes. Due to late apoptotic or necrotic cell membrane loss of integrity, 7-AAD can enter cells to stain DNA and, when used in combination with Annexin V, distinguish cells at different stages of apoptosis.