Rat Shh (Sonic hedgehog protein) QuickTest ELISA Kit

Category:

This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains Shh, a capture antibody-Shh-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Shh in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
QT-ER0601
Alias: 
Sonic hedgehog protein ELISA Kit , SHH ELISA Kit , HHG-1 ELISA Kit , Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains ELISA Kit , ShhNC ELISA Kit , Sonic hedgehog protein N-product ELISA Kit , ShhN ELISA Kit , Shh N-terminal processed signaling domains ELISA Kit , ShhNp ELISA Kit , SHH ELISA Kit
Gene Name: 
Shh
UniProt ID: 
Q63673
MSDS
Size Price
48T/96T Inquiry
Dispatch Time: About 3 working days
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Product Name
Rat Shh (Sonic hedgehog protein) QuickTest ELISA Kit
Catalogue No.
QT-ER0601
Gene Name
Shh
UniProt ID
Q63673
Alias
Sonic hedgehog protein ELISA Kit , SHH ELISA Kit , HHG-1 ELISA Kit , Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains ELISA Kit , ShhNC ELISA Kit , Sonic hedgehog protein N-product ELISA Kit , ShhN ELISA Kit , Shh N-terminal processed signaling domains ELISA Kit , ShhNp ELISA Kit , SHH ELISA Kit
Sample Type
Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
120 minutes
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Specificity
Specifically binds with Shh , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
E001ELISA Microplate(Dismountable) 8×68×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002Lyophilized Standard 1vial2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E054Cap/Det Ab (Ready to use, blue) 3ml6ml 2-8°C (Avoid Direct Light)
E053HRP-Streptavidin (Ready to use, orange) 5ml10ml
E024TMB Substrate 5ml10ml
E039Sample Dilution Buffer 20ml20ml 2-8°C
E026Stop Solution 5ml5ml
E038Wash Buffer(25X) 15ml30ml
E006Plate Sealer 3 pieces5 pieces
E007Product Description 1 copy1 copy
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
00.1040.1080.106
0.1560.1710.1690.171
0.3120.3850.3750.38
0.6250.8040.7980.769
1.251.0871.0791.032
2.51.3261.3361.363
51.8931.9111.861
102.4362.3782.395
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (ng/ml)0.311.245.340.31
Inter-assay Precision Standard deviation0.010.040.20.01
Inter-assay Precision CV(%)4.13.563.794.08


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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